Reactivity of the tryptophan residues in bovine pancreatic deoxyribonuclease with N-bromosuccinimide.

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Reactivity of the tryptophan residues in bovine pancreatic deoxyribonuclease with N-bromosuccinimide.

DNase, containing 3 tryptophan residues, can be inactivated by modification of this amino acid with Nbromosuccinimide. Chromatography of acid hydrolysates shows all three tryptophan residues modified by the time inactivation is complete. However, spectrophotometric measurement indicates that only 2 residues are modified. The discrepancy is due to the unreliability of the spectrophotometric meth...

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Bovine pancreatic deoxyribonuclease D.

Of the four deoxyribonucleases, DNases A, B, C, and D, observable by chromatography of preparations of the pancreatic enzyme on cellulose phosphate, DNase D, the component present in the smallest amount, has not been previously characterized. Peptide maps show that the sequence of the amino acid residues in the polypeptide chain of DNase D is indistinguishable from that of DNase C; analysis of ...

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The carbohydrate moiety of bovine pancreatic deoxyribonuclease.

Preceding experiments by Price and his associates establish that bovine pancreatic deoxyribonuclease is a glycoprotein which contains glucosamine and mannose. In order to determine the mode of attachment of the carbohydrate to the enzyme molecule, chromatographically purified deoxyribonuclease has been digested with Pronase. Gel filtration of the digest yielded a fraction containing all of the ...

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Properties of chromatographically purified bovine pancreatic deoxyribonuclease.

Preparations of bovine pancreatic deoxyribonuclease obtained by the ammonium sulfate precipitation procedure of Kunitz can be further purified by chromatography on sulfoethyl-Sephadex at pH 4.70. The instability of earlier preparations of the enzyme has usually been attributed to the sensitivity of the protein to proteolytic digestion. This hypothesis has been confirmed; treatment of the starti...

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Comparison of the multiple forms of bovine pancreatic deoxyribonuclease.

The earlier resolution of preparations of bovine pancreatic deoxyribonuclease into two active fractions by chromatography on sulfoethyl-Sephadex has been improved through the use of columns of phosphocellulose. Gradient elution with sodiwn acetate at pH 4.7 shows the presence of at least four forms of the enzyme. The principal component (DNase A) is accompanied by Fractions B and C, and, in ver...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1980

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)43546-6