Reactivity of the tryptophan residues in bovine pancreatic deoxyribonuclease with N-bromosuccinimide.
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منابع مشابه
Reactivity of the tryptophan residues in bovine pancreatic deoxyribonuclease with N-bromosuccinimide.
DNase, containing 3 tryptophan residues, can be inactivated by modification of this amino acid with Nbromosuccinimide. Chromatography of acid hydrolysates shows all three tryptophan residues modified by the time inactivation is complete. However, spectrophotometric measurement indicates that only 2 residues are modified. The discrepancy is due to the unreliability of the spectrophotometric meth...
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Preparations of bovine pancreatic deoxyribonuclease obtained by the ammonium sulfate precipitation procedure of Kunitz can be further purified by chromatography on sulfoethyl-Sephadex at pH 4.70. The instability of earlier preparations of the enzyme has usually been attributed to the sensitivity of the protein to proteolytic digestion. This hypothesis has been confirmed; treatment of the starti...
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The earlier resolution of preparations of bovine pancreatic deoxyribonuclease into two active fractions by chromatography on sulfoethyl-Sephadex has been improved through the use of columns of phosphocellulose. Gradient elution with sodiwn acetate at pH 4.7 shows the presence of at least four forms of the enzyme. The principal component (DNase A) is accompanied by Fractions B and C, and, in ver...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43546-6